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In 1910, A. L. Mills established the Northwestern Garment Factory, making aprons for stock yards. In 1912, he established Mills Hospital Supplies Inc., making surgical gowns and nurses uniforms for hospitals. In the 1920s, his son Irving took over the business and began distributing general medical supplies in addition to the garments. In 1961, Irving sold the company to Cenco. In 1966, A. L.’s grandsons Jim and Jon Mills left Cenco and founded Medline with approximately 12,000 square feet of warehouse space and one loading dock in Evanston, Illinois In 1968, Medline opened its first textile manufacturing facility in Covington, Indiana. In 1972, Medline opened its first non-textile manufacturing division (Dynacor) with the purchase of an injection molding company.
In 1995, Medline opened its first facility for assembling sterile procedure trays (SPTs), in Waukegan, Illinois. In 1996, Medline entered the distribution business. In 2001, Medline started manufacturing private label wound care products for CVS, Walgreens, Target, Dollar General and Family Dollar.
In April 2007, Medline acquired the Curad brand of first-aid products from Beiersdorf, entering the retail market. The Curad brand includes products such as bandages, surgical tape, and liquid bandage.
In 2009, Medline Launched Generation Pink exam gloves to help raise breast cancer awareness and turned the original Pink Glove Dance video into a viral breast cancer awareness campaign, reaching 14+ million people. In 2011, Medline introduced BioMask, the first-ever FDA-cleared antiviral medical facemask shown to inactivate flu viruses.
As of 2016, Medline offers over 550,000 medical products and clinical solutions to hospitals, extended care facilities, surgery centers, physician offices, home care agencies and providers, and retailers. It has over 35,000 employees in over 125 countries and territories (such as the USA, Canada, Mexico, Spain, Germany, the United Kingdom, France, Italy, Japan, Australia and New Zealand) and include clinicians, researchers, engineers, financial experts and 2,000 direct sales representatives. Medline operates over 50 distribution centers across North America.
In October 2023, Jim Boyle became Medline CEO. In December 2025, Medline completed an initial public offering, listing on Nasdaq at $29 per share, raising more than $6.26 billion and valuing the company at over $37 billion.
On June 11, 2026, a fire destroyed a Medline distribution center in Tracy, California. The incident was reported to be one of the largest warehouse fires in the history of the United States and the building was deemed a total loss. The cause of the fire remains under investigation.